By R.H. BURDON and P.H. van KNIPPENBERG (Eds.)
This quantity is a complete selection of equipment for the isolation, characterization, research and estimation of soluble lipoproteins. It surveys each one element of lipoprotein expertise in a serious demeanour so that it will allow the investigator to choose the equipment so much suitable to his standards. Having made his selection, he'll then discover a distinctive description of the way to accomplish the procedure.
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7. However, although these are the rotors to which we shall refer in this manual, the equivalent products of other manufacturers can also be used satisfactorily. The actual choice of rotor is determined by several factors. It must be remembered that the purpose of the centrifugation is not only to float the required lipoproteins to the top of the tube but also to sediment the plasma proteins an adequate distance towards the bottom. In the case of VLDL, the density of both the lipoproteins and the plasma proteins differ from that of the solvent by enough to allow them to be almost completely separated by a few hours centrifugation at a relatively low speed.
1765 g/ml is therefore fairly close to the actual density of the required diluent. Note that, if the amount by which the density of the original solution is to be raised is smaller than in this example, the first approximation will often be within limits. 0934 g/ml and this requires no further refinement. OO02 g/ml and a refined estimate for the density of the diluent must be calculated as follows: (D) First, estimate the real volume of the mixture that results when the diluent is added to the plasma, by putting into eqn.
7600 1. 35761 rlzo nu Ch. 3892. 2 the value of C,and C, for plasma and for the final, adjusted mixture. 33 mg/ml of water. The total weight of NaCl in the final mixture is therefore given by eqn. e. 86 mg/mI. Likewise, from eqn. 93 mg/ml. 2 will show that these two values do not correspond to a single salt solution. 3 The properties of sodium bromide solutions. 2. Mol. wt. 9925 Ch. 3077) is a close approximation to that required. 1765 g/ml. 3046) mg/ml (C) From eqns. e. 2064. 1248 g/ml, which is almost within the acceptable tolerance limits.