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39 40 42 46 47 References . . . . . . . . . . . . . . . . . . 48 Advances in Biochemical Engineering/ Biotechnology, Vol. 73 Managing Editor: Th. Scheper © Springer-Verlag Berlin Heidelberg 2001 32 R. McDaniel et al. 1 Introduction Polyketides, a large family of bioactive natural products, have found considerable use in human health and agriculture. Their structural complexity necessitates that they be produced via fermentation routes.
34 5 Classical Strain Improvement for Polyketide Production . . . 34 6 Process Development for Polyketide Production . . . . . . 3 Clone Selection and Culture Preservation . . . . . . . . 35 Media Development . . . . . . . . . . . . . . 36 Small-Scale Fermentation . . . . . . . . . . . . 37 7 Metabolic Engineering for the Overproduction of Polyketides . . 5 Heterologous Expression of Polyketides . . . . Maximizing Gene Expression . . . . . . .
3 Clone Selection and Culture Preservation . . . . . . . . 35 Media Development . . . . . . . . . . . . . . 36 Small-Scale Fermentation . . . . . . . . . . . . 37 7 Metabolic Engineering for the Overproduction of Polyketides . . 5 Heterologous Expression of Polyketides . . . . Maximizing Gene Expression . . . . . . . Enhancing Precursor Supplies . . . . . . Superhosts . . . . . . . . . . . . Genomics Guided Process and Strain Improvement 8 Conclusion .